Analysis of Protein Carbonyl by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1351-LGZ)

The carbonyl content of the oxidized protein increased, and the carbonyl group reacted with 2, 4-dinitrophenylhydrazide to form a reddish-brown precipitate. After the precipitate was dissolved, the absorbance value at 370 nm could be read on the spectrophotometer to calculate the carbonyl content of the protein.

Used to detect the content of protein carbonyl in serum, plasma, pleural fluid, cell supernatant and tissue samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum, plasma, pleural fluid, cell supernatant and tissue samples