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Analysis of β - galactosidase (β –GAL) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1389-LGZ)
Introduction
Sensitivity: 16.19 U/L
Detection range: 16.19-1200.0 U/L
Precision: inter-lot difference of 6%, intra-lot difference of 3%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 390-410 nm
Principle
β-galactosidase can catalyse substrate PNPG (p-nitrophenyl-β-D-galactosidine) to p-nitrophenol. P-nitrophenol has a maximum absorption peak at 400 nm. The enzyme activity of β-GAL can be calculated by measuring the change of absorbance value in unit time.
Applications
It is suitable for the detection of β-galactosidase activity in serum (plasma), animal and plant tissues and cell samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: serum, plasma, animal and plant tissues and cell samples
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