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Analysis of Plant Flavonoids by Enzyme-labeled Instrument (CAT#: STEM-MB-1425-LGZ)
Introduction
Sensitivity: 0.66 -μg/mL
Detection range: 0.66 - 150 μg/mL
Precision: inter-lot difference of 5.3%, intra-lot difference of 4%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 500-520 nm
Principle
In the alkaline nitrite solution, flavonoids and aluminum ions form red complex, determination of the sample extract at 510 nm light absorption value, can calculate the flavonoid content of the sample.
Applications
Used to detect flavonoid content in plant tissue samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: plant tissue samples
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Wide range of applications
For various laboratory needs
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