Analysis of Alkaline Phosphatase (ALP) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1340-LGZ)

Alkaline phosphatase catalyzes the hydrolysis of phenyldisodium phosphate to produce free phenol and phosphate in the reaction solution at pH=10. Phenol is combined with 4-amino-antipyrine in alkaline solution, and the red quinone derivatives are oxidized by potassium ferricyanide. The level of enzyme activity is calculated according to the shade of red.

Used to detect the activity of alkaline phosphatase in animal serum (plasma), body fluids, tissues and cells.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: animal serum (plasma), body fluids, tissues and cells