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Analysis of Copper (Cu) by Enzyme-labeled Instrument (CAT#: STEM-MB-1381-LGZ)
Introduction
Sensitivity: 1.84 μmol/L
Detection range: 1.84-60 μmol/L
Precision: inter-lot difference of 3.1%, intra-lot difference of 3%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 575-585 nm
Principle
Under acidic conditions, copper ions dissociate from ceruloplasmin and react with complexing agent 3, 5-Dibr-Paesa to produce purple complexes with absorbance values proportional to concentration within a certain range.
Applications
Applicable to the detection of copper ion content in serum, plasma and animal tissue samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: serum, plasma and animal tissue samples
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For various laboratory needs
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