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Analysis of Plant Flavonoids by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1344-LGZ)
Introduction
Sensitivity: 0.315 μg/mL
Detection range: 0.315-150 μg/mL
Precision: inter-lot difference of 2.2%, intra-lot difference of 1.9%
Detection equipment: Ultraviolet-visible Spectrophotometer
Detection wavelength: 510 nm
Principle
In the alkaline nitrite solution, flavonoids and aluminum ions form red complex, determination of the sample extract at 510 nm light absorption value, can calculate the flavonoid content of the sample.
Applications
It is suitable for the determination of flavonoids in plant tissue samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: plant tissues
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