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Analysis of Total Phenols by Enzyme-labeled Instrument (CAT#: STEM-MB-1436-LGZ)
Introduction
Sensitivity: 1.05 μg/mL
Detection range: 1.05–148 μg/mL
Precision: inter-lot difference of 4.4%, intra-lot difference of 4.1%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 750-770 nm
Principle
Under alkaline conditions, phenolic substances reduce tungstate molybdate to produce blue compounds. The extraction solution of the test sample has the maximum absorption at 760 nm, and its color depth is proportional to the total phenolic content. The total phenolic content of the sample can be calculated by colorimetry.
Applications
For the determination of total phenolic content in plant tissue samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: plant tissue samples
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For various laboratory needs
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