Analysis of Total Antioxidant Status (TAS) by Enzyme-labeled Instrument (CAT#: STEM-MB-1448-LGZ)

ABTS are oxidized into green ABTS+ under the action of appropriate oxidants, and ABTS+ is reduced to colorless ABTS in the presence of antioxidants. The total antioxidant capacity in samples can be measured and calculated by measuring the absorbance change of ABTS+ at 660 nm wavelength. Trolox is a vitamin E analogue with similar antioxidant capacity as vitamin E and is used as a reference for total antioxidant capacity.

Used for the determination of total antioxidant status (TAS) in serum (plasma), urine, animal and plant tissues, and cell supernatant samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum (plasma), urine, animal and plant tissues, and cell supernatant samples