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Analysis of Phosphate by Enzyme-labeled Instrument (CAT#: STEM-MB-1487-LGZ)
Introduction
Sensitivity: 0.001 mmol/L
Detection range: 0.001 -0.20 mmol/L
Precision: inter-lot difference of 8%, intra-lot difference of 4%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 630 - 650 nm
Principle
The compounds formed by phosphate radical and molybdic acid radical under acidic conditions will form color substances with malachite green. The depth of color is proportional to the content of phosphate. Phosphate content in samples can be determined by measuring the absorbance change at 636 nm.
Applications
Used to detect phosphate content in serum, plasma, tissue and cell samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: serum, plasma, tissue and cell samples
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