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Analysis of Malondialdehyde (MDA) by Enzyme-labeled Instrument (CAT#: STEM-MB-1372-LGZ)
Introduction
Sensitivity: 0.52 nmol/mL
Detection range: 0.52-120 nmol/mL
Precision: inter-lot difference of 5.1% , intra-lot difference of 5.1%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 525-535 nm
Principle
In the alkaline nitrite solution, flavonoids and aluminum ions form red complexes, and the content of flavonoids in the samples can be calculated by measuring the absorbance of the sample extract at 510 nm.
Applications
It is suitable for the determination of MDA content in plant tissues.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: plant tissues
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