High-quality products
Reliable performance
Analysis of Catalase (CAT) Activity by Fluorescence Microplate Reader (CAT#: STEM-MB-1501-LGZ)
Introduction
Sensitivity: 0.01 U/L
Detection range: 0.01 -6.51 U/L
Precision: inter-lot difference of 6.8%, intra-lot difference of 3.4%
Detection equipment: Fluorescence Microplate Reader
Excitation wavelength: 535 nm
Emission wavelength: 587 nm
Principle
In the detection system, catalase decomposes H2O2 to produce water and oxygen. The undecomposed hydrogen peroxide reacts in the presence of enzyme and fluorescent substance, and its fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 587 nm is proportional to the hydrogen peroxide concentration.
Applications
It is suitable for detecting catalase activity in serum (plasma), animal and plant tissue samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Determination of fluorescence intensity.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Determination of fluorescence intensity.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: serum (plasma), animal and plant tissue samples
Other recommended products
-
Analysis of Peroxidase (POD) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1434-LGZ)
-
Analysis of NAD-Malate Dehydrogenase (NAD-MDH) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1464-LGZ)
-
Analysis of Inhibition and Production of Superoxide Anionic by Enzyme-labeled Instrument (CAT#: STEM-MB-1412-LGZ)
-
Analysis of β-Amylase Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1415-LGZ)
-
Analysis of Citric Acid (CA) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1359-LGZ)
-
-
Analysis of Total Iron Binding Capacity (TIBC) by Enzyme-labeled Instrument (CAT#: STEM-MB-1411-LGZ)
-
Analysis of Total Carbonyl by Enzyme-labeled Instrument (CAT#: STEM-MB-1399-LGZ)
Wide range of applications
For various laboratory needs
Expert support
Professional service team
Fast delivery
Global shipping