Analysis of Lactate Dehydrogenase (LDH) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1366-LGZ)

With coenzyme I as the hydrogen delivery body, LDH catalyzes lactic acid to produce pyruvate, pyruvate and 2, 4-dinitrophenylhydrazide to produce pyruvate dinitrophenylhydrazone, the latter in alkaline solution is brown red, the color of the color is proportional to the concentration of pyruvate, through the determination of OD value, LDH activity can be calculated.

Suitable for detection of LDH activity in serum, plasma, tissue, cell and other samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum, plasma, tissue, cell and other samples