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Analysis of Lactase Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1422-LGZ)
Introduction
Sensitivity: 3.94 U/ mL
Detection range: 12.5-2000 U/ mL
Precision: inter-lot difference of 8.5%, intra-lot difference of 4.5%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 495 -510 nm
Principle
Lactase breaks down lactose to produce glucose, which, under the action of the enzyme, produces hydrogen peroxide. In the presence of chromogenic oxygen receptors, peroxidase catalyzes hydrogen peroxide to oxidize pigment sources and produce colored substances.
Applications
It applies to lactase activity in animal tissue samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: animal tissue samples
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For various laboratory needs
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