Analysis of Lactate Dehydrogenase (LDH) by Enzyme-labeled Instrument (CAT#: STEM-MB-1441-LGZ)

Lactate dehydrogenase catalyzes the reaction of lactic acid and NAD+ to produce pyruvate and NADH. Under the action of PMS, NADH transfers electrons to WST-8 to produce yellow products with characteristic absorption peaks at 450 nm.

It was used to detect the activity of lactate dehydrogenase in animal tissue samples, serum (plasma), pleural fluid and cell samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: animal tissue samples, serum (plasma), pleural fluid and cell samples