Analysis of Xanthine Oxidase (XOD) Activity by Fluorescence Microplate Reader (CAT#: STEM-MB-1503-LGZ)

Hypoxanthine can be oxidized by xanthine oxidase (XOD) to produce xanthine and superoxide anion, which is quickly converted into hydrogen peroxide in the system. Under the action of peroxidase, hydrogen peroxide oxidizes the non-fluorescent probe into a fluorescent substance. By measuring the fluorescence value of the system, the activity of the corresponding xanthine oxidase can be calculated.

It is suitable for the determination of xanthine oxidase activity in serum, plasma and animal tissue samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Determination of fluorescence intensity.
4. Make the mark curve and calculate the result.

• Sample Type: serum, plasma and animal tissue samples