Analysis of Glycogen by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1322-LGZ)

In the presence of concentrated sulfuric acid, the glycogen can be dehydrated to produce furfural derivatives, which form blue compounds with anthranone, and the standard solution of glucose treated by the same method at 620 nm wavelength. Glycogen is very stable in a concentrated alkali solution, so the tissue is heated in the concentrated alkali solution prior to color development to destroy other components and retain glycogen.

It is suitable for detecting glycogen content in liver and muscle of animal.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: Animal liver, muscle