Analysis of L-Lactic Acid (LA) by Enzyme-labeled Instrument (CAT#: STEM-MB-1393-LGZ)

Using oxidized coenzyme Ⅰ (NAD+) as hydrogen receptor, lactate dehydrogenase (LDH) catalyzes lactate dehydrogenation to produce pyruvate, which converts NAD+ into NADH. N-methylphenazine methyl sulfate (PMS) dehydrogenated nitrotetrazazole blue (NBT) chloride to purple color, color absorbance at 530 nm and the content of lactic acid showed a linear relationship.

Applicable to the detection of lactic acid content in serum (plasma), cell supernatant, tissue, cell and other samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum, plasma, cell supernatant, tissue, cell and other samples