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Analysis of Vitamin E (VE) by Enzyme-labeled Instrument (CAT#: STEM-MB-1402-LGZ)
Introduction
Sensitivity: 0.95 μg/mL
Detection range: 0.95-40 μg/mL
Precision: inter-lot difference of 6.3%, intra-lot difference of 3.9%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 525-533 nm
Principle
Vitamin E, in the presence of phenanthroline, reduces ferric ions to ferric ions, which under certain circumstances can form pink complexes with phenanthroline, with a maximum absorption wavelength at 533 nm. Vitamin E content can be calculated by colorimetry.
Applications
It is suitable for the determination of vitamin E(VE) in animal serum (plasma), animal and plant tissues.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: serum, plasma, animal and plant tissues
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For various laboratory needs
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