Analysis of Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) by Enzyme-labeled Instrument (CAT#: STEM-MB-1397-LGZ)

GSSG is reduced to GSH by glutathione reductase, and GSH can react with color substrate DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus total glutathione can be calculated by measuring A412. Then the GSH in the sample is removed with appropriate reagents and the GSSG content can be determined by using the reaction principle above.

For the determination of total glutathione (T-GSH) and oxidized glutathione (GSSG) in serum (plasma), animal tissues, red blood cells and cultured cells.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum, plasma, animal tissues, red blood cells and cultured cells