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Analysis of Uric Acid (UA) by Enzyme-labeled Instrument (CAT#: STEM-MB-1401-LGZ)
Introduction
Sensitivity: 1.30 mg/L
Detection range: 1.30-80 mg/L
Precision: inter-lot difference of 4%, intra-lot difference of 2%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 680-700 nm
Principle
Phosphotungstic acid reduction method uses uric acid (UA) in protein-free filtrate to reduce phosphotungstic acid to produce tungstic blue, allantoin and carbon dioxide under alkaline conditions. The color shade is proportional to the concentration of uric acid.
Applications
It is suitable for the determination of uric acid in serum, plasma and urine samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: serum, plasma, urine
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