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Analysis of Total Carbonyl by Enzyme-labeled Instrument (CAT#: STEM-MB-1399-LGZ)
Introduction
Sensitivity: 1.29 μg/mL
Detection range: 1.29-45 μg/mL
Precision: inter-lot difference of 5%, intra-lot difference of 1.9%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 365-375 nm
Principle
Carbonyl can react with phenylhydrazine to form a hydrazone, which has a characteristic absorption peak at 370 nm. The content of carbonyl can be calculated by colorimetric determination of absorbance value.
Applications
It is suitable for the determination of total carbonyl in serum, plasma and animal and plant tissue samples.
Procedure
1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.
Materials
• Sample Type: serum, plasma, urine
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