Amplicon Sequencing (CAT#: STEM-MB-0047-WXH)

Introduction

Amplicon sequencing is the sequencing of PCR products or captured fragments of a specific length. 16S/18S/ITS and other amplicon sequencing is to extract the DNA of environmental samples, select appropriate universal primers to amplify the target region of 16S/18S/ITS, and detect the sequence variation and abundance of the target region, to study environmental microbial diversity and community composition differences. 16S/18S rDNA is the DNA sequence encoding proto/eukaryotic ribosomal small subunit rRNA. ITS is divided into two regions: ITS1 is located between 18S and 5.8S in the eukaryotic DNA sequence, and ITS2 is located between 5.8S and 28S.