Methylated DNA Binding Domain Sequencing (MBD-Seq) to analyze DNA Methylation (CAT#: STEM-MB-0051-WXH)

Introduction

Methylated DNA Binding Domain-Sequencing (MBD-Seq) is the use of proteins that specifically bind to highly methylated DNA to enrich highly methylated DNA, and then use high-throughput sequencing technology to sequence the enriched DNA method. To detect methylation sites on a genome-wide scale. This technology can specifically detect methylated modified regions in the genome, and is suitable for studies on genetic imprinting, embryonic development, and tumor development.

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Applications

Epigenetic studies of genetic imprinting, embryonic development, tumorigenesis, etc.

Procedure

1.DNA sample preparation
2.Isolation of methylated DNA by recombinant MBD
3.Library preparation and sequencing

Notes

Customer provides tissue, cell, DNA and other samples and related information

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