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TNF-α Detection (CAT#: STEM-MB-0305-WXH)
Introduction
Tumour Necrosis Factor alpha (TNF alpha), is an inflammatory cytokine produced by macrophages/monocytes during acute inflammation and is responsible for a diverse range of signalling events within cells, leading to necrosis or apoptosis. The protein is also important for resistance to infection and cancers.
Principle
As a cytokine, TNF needs to bind to cell surface receptors to complete the transmission of cellular signals. After years of human exploration and research results, human TNF receptors (TNFR) can be divided into two categories: TNFR1 and TNFR2，they are type I transmembrane proteins. TNFR1 is widely distributed on most cell surfaces in vivo, and the expression of TNFR2 is mainly localized on the surface of immune cells.
Applications
TNF-α is responsible for a diverse range of signalling events within cells, leading to necrosis or apoptosis.
Procedure
1. Process samples.
2. TNF-α detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.
2. TNF-α detection (qPCR, Enzyme-linked immunosorbent assay (ELISA), Flow cytometry).
3. Analysis results.
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