High-quality products
Reliable performance
Spectral karyotype analyses of cartilage tumours (CAT#: STEM-ST-0381-WXH)
Introduction
Cartilage tumors vary in severity from benign enchondroma to low-grade malignant chondrosarcoma to the highest-grade dedifferentiated chondrosarcoma. Chondrosarcoma is the second most common primary malignant bone tumor, accounting for 10-20% of all primary bony malignancies.
Principle
It is based on the hybridization of collections of chromosome-specific FISH probes labeled with different fluorochromes or fluorochrome combinations, allowing the discrimination of each of the 24 different human chromosomes.
Applications
Detection of subtle chromosomal aberrations, including marker chromosomes, small translocations, complex rearrangements and minute structural abnormalities.
Procedure
1. Metaphase preparation
2. Slide pretreatment
3. Slide and probe denaturation
4. Hybridization
5. Detection
6. Image acquisition and image analysis
2. Slide pretreatment
3. Slide and probe denaturation
4. Hybridization
5. Detection
6. Image acquisition and image analysis
Materials
Fluorescent dyes, spectrum orange, Texas red, Cy5, spectrum green, Cy5
Other recommended products
-
Characterization of Chromosomal Rearrangements Using Spectral Karyotyping (CAT#: STEM-ST-0382-WXH)
-
Characterization of chromosomal abnormalities in prostate cancer cell lines by spectral karyotyping (CAT#: STEM-ST-0378-WXH)
-
Spectral Karyotype (SKY) Technique (CAT#: STEM-ST-0370-WXH)
-
-
Characterization of HeLa Cells by Spectral Karyotyping (CAT#: STEM-ST-0380-WXH)
-
Multicolour spectral karyotyping of mouse chromosomes (CAT#: STEM-ST-0371-WXH)
-
Analysis of B-cell Neoplasias by Spectral Karyotyping (SKY) (CAT#: STEM-ST-0383-WXH)
-
Study on human hepatocellular carcinoma by spectral karyotyping (CAT#: STEM-ST-0374-WXH)
Wide range of applications
For various laboratory needs
Expert support
Professional service team
Fast delivery
Global shipping