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Characterization of HeLa Cells by Spectral Karyotyping (CAT#: STEM-ST-0380-WXH)
Introduction
HeLa is an immortalized cell line used in scientific research. It is the oldest human cell line and one of the most commonly used. HeLa cells are durable and prolific, allowing for extensive applications in scientific study.
Principle
It is based on the hybridization of collections of chromosome-specific FISH probes labeled with different fluorochromes or fluorochrome combinations, allowing the discrimination of each of the 24 different human chromosomes.
Applications
Detection of subtle chromosomal aberrations, including marker chromosomes, small translocations, complex rearrangements and minute structural abnormalities.
Procedure
1. Metaphase preparation
2. Slide pretreatment
3. Slide and probe denaturation
4. Hybridization
5. Detection
6. Image acquisition and image analysis
2. Slide pretreatment
3. Slide and probe denaturation
4. Hybridization
5. Detection
6. Image acquisition and image analysis
Materials
Fluorescent dyes, spectrum orange, Texas red, Cy5, spectrum green, Cy5
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Wide range of applications
For various laboratory needs
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