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Study on human hepatocellular carcinoma by spectral karyotyping (CAT#: STEM-ST-0374-WXH)
Introduction
The most common type of liver cancer starts in cells called hepatocytes and is called hepatocellular carcinoma. Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer.
Principle
It is based on the hybridization of collections of chromosome-specific FISH probes labeled with different fluorochromes or fluorochrome combinations, allowing the discrimination of each of the 24 different human chromosomes.
Applications
Detection of subtle chromosomal aberrations, including marker chromosomes, small translocations, complex rearrangements and minute structural abnormalities.
Procedure
1. Metaphase preparation
2. Slide pretreatment
3. Slide and probe denaturation
4. Hybridization
5. Detection
6. Image acquisition and image analysis
2. Slide pretreatment
3. Slide and probe denaturation
4. Hybridization
5. Detection
6. Image acquisition and image analysis
Materials
Fluorescent dyes, spectrum orange, Texas red, Cy5, spectrum green, Cy5
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