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Spectral Karyotype (SKY) Technique (CAT#: STEM-ST-0370-WXH)
Introduction
Spectral karyotype (SKY) is a karyotype in which the homologous pairs of chromosomes are manipulated in such a way that they have distinctive colors. The SKY technique makes it easier for scientists to detect chromosomal abnormalities, as compared with a conventional karyotype.
Principle
It is based on the hybridization of collections of chromosome-specific FISH probes labeled with different fluorochromes or fluorochrome combinations, allowing the discrimination of each of the 24 different human chromosomes.
Applications
Detection of subtle chromosomal aberrations, including marker chromosomes, small translocations, complex rearrangements and minute structural abnormalities.
Procedure
1. Metaphase preparation
2. Slide pretreatment
3. Slide and probe denaturation
4. Hybridization
5. Detection
6. Image acquisition and image analysis
2. Slide pretreatment
3. Slide and probe denaturation
4. Hybridization
5. Detection
6. Image acquisition and image analysis
Materials
Fluorescent dyes, spectrum orange, Texas red, Cy5, spectrum green, Cy5
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For various laboratory needs
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