Recombinant Murine TNF α Protein, Seafrom (CAT#: STEM-CT-0041-ZJF)

The Recombinant Murine TNF α Protein manufactured by Seafrom is suitable for pro-inflammatory effects and immune response regulation.
Tumor Necrosis Factor-Alpha (TNF-α) is a pleiotropic pro-inflammatory cytokine secreted by various cell types, including adipocytes, activated monocytes, macrophages, B cells, T cells, and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α exhibits cytotoxicity against various tumor cells and plays a critical role in mediating immune responses to bacterial infections. It is involved in septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes induction.
The lyophilized product can be stored at 2-8°C for 24 months. For reconstitution, it can be dissolved in injectable water, saline solution, culture medium, or PBS. After dissolution, it should be aliquoted, with a recommended storage period of 6 months at -20°C and 12 months at -80°C. When diluting, ensure a final concentration of no less than 100 μg/mL and avoid repeated freeze-thaw cycles.

Expression Host: HEK293 Cells
Synonyms: Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin
Protein Sequence: DNA sequence encodes mouse TNF-α (NP_038721.1) with a His tag at the C-terminus, predicted molecular weight of 18.9 kD
Purity: >90%, analyzed by SDS-PAGE
Endotoxin Level: <1 EU/μg
Biological Activity: Lysis capability determined using mouse L929 cells in the presence of actinomycin D, ED50 is 20-100 pg/mL
Purification Method: Chromatographic purification
Appearance: White loose body

Stable Cell Library
Large-Scale Expression
High Activity
Batch Stability
Gmp-Quality Control
Ensured Product Quality

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