Recombinant SARS-CoV-2 Spike Protein (S1 Subunit), Seafrom (CAT#: STEM-CT-0107-ZJF)

The Recombinant SARS-CoV-2 Spike Protein (S1 Subunit) manufactured by Seafrom is suitable for studying SARS-CoV-2 virus-host interactions.
The spike (S) glycoprotein of coronaviruses contains protrusions that specifically bind to certain receptors on the host cell. Known receptors that bind to the S1 subunit of the spike protein include ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), and CEACAM (carcinoembryonic antigen-related cell adhesion molecule). The spike protein is essential for both host specificity and viral infectivity.
The spike (S) glycoprotein of coronaviruses is known to be crucial for the initial binding of the virus to the host cell during the infection process. It has been reported that SARS-CoV-2 can infect human respiratory epithelial cells by interacting with the human ACE2 receptor. The spike protein is a large type I transmembrane protein consisting of two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), responsible for recognizing the cell surface receptor, while S2 contains basic elements required for membrane fusion.
The S protein plays a significant role in inducing neutralizing antibody and T-cell responses, contributing to protective immunity. The main functions of the Spike protein are summarized as follows:
1. Mediating receptor binding and membrane fusion.
2. Defining the range of hosts and the virus's specificity.
3. Serving as a primary target for binding with neutralizing antibodies.
4. Being a key target for vaccine design.
5. The potential for transmission between different hosts through gene recombination or mutation of the receptor binding domain (RBD), which could lead to a higher mortality rate.
Storage Information:
1. Lyophilized samples can be stored at 4°C for 24 months, and reconstituted liquid can be stored at -20°C for 6-12 months, while avoiding repeated freeze-thaw cycles.
2. Liquid samples can be stored at -20 to -80°C for 12 months, and after thawing, should be aliquoted and stored at -20°C for an additional 6-12 months, while avoiding repeated freeze-thaw cycles.

Synonyms: 2019-nCoV coronavirus spike Protein; 2019-nCoV cov spike Protein; ncov RBD Protein, 2019-nCoV;2019-nCoV ncov s1 Protein; 2019-nCoV NCP-CoV s1 Protein
Expression Host: Human HEK293 cells
Protein Sequence: The DNA sequence encodes recombinant SARS-CoV-2 (2019-nCoV) S1 protein, expressed with a His tag at the C-terminus.
Molecular Weight: Recombinant SARS-CoV-2 (2019-nCoV) S1 protein contains 698 amino acids, with a predicted theoretical molecular weight of 78.3 kD.
Purity: >90%, confirmed by SDS-PAGE gel analysis.
Endotoxin: ≤0.01 EU/μg (gel-clot method)
Purification Method: Chromatographic purification
Composition:
Freeze-dried powder of sterile PBS (pH 7.4) containing 6% mannitol and 6% trehalose.
Liquid of sterile PBS (pH 7.4) filtered through a 0.2 μM filter at a concentration of 0.2 μM.

Optimal Stability
Gmp-Grade Quality
Low Batch-to-Batch Variability
Exceptional Specificity
Robust Bioactivity
Efficient and Reliable Performance

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