Recombinant Human LIF Protein, Seafrom (CAT#: STEM-CT-0065-ZJF)

The Recombinant Human LIF Protein manufactured by Seafrom is suitable for cell proliferation, differentiation, and survival.
LIF (Leukemia Inhibitory Factor) is a pleiotropic factor produced by various cell types, including T cells, myeloid lineage, fibroblasts, liver, heart, and melanomas. LIF plays a role in inhibiting the spontaneous differentiation of embryonic stem cells and promoting their long-term maintenance. Other activities of LIF include stimulating the acute-phase protein synthesis in liver cells, promoting the differentiation of cholinergic neurons, and inhibiting fat cell development by suppressing lipoprotein lipase.
Human LIF is active on murine cells and is widely used for maintaining the spontaneous differentiation of mouse embryonic stem cells. Mouse LIF cannot exert its effects on human cells as it cannot bind to human LIF receptors. Recombinant human LIF is a 19.6 kDa protein composed of 180 amino acid residues and contains three disulfide bonds.
The lyophilized formulation can be stored at 4°C for 24 months. Once reconstituted, the liquid can be stored at -20°C for 6-12 months, while avoiding repeated freeze-thaw cycles.

Expression Host: HEK293 Cells
Synonyms: CDF, DIA, HILDA, Leukemia Inhibitory Factor, MLPLI
Protein Sequence: DNA sequence encodes human LIF (NP_002300.1) with a His tag at the C-terminus, contains 186 amino acids, predicted molecular weight of 20.5 kD
Purity: ≥95%, analyzed by SDS-PAGE and HPLC
Endotoxin Level: ≤0.01 EU/μg (gel clot method)
Biological Activity: Depends on its ability to stimulate human TF-1 cell proliferation, expected ED50 is ≤0.1 ng/mL, corresponding to ≥1×10^7 U/mg specific activity
Purification Method: Chromatographic purification

Stable Cell Library
Large-Scale Expression
High Activity
Batch Stability
Gmp-Quality Control
Ensured Product Quality

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