Recombinant Human Her3/ErbB3 (hFc Tag), Yisheng (CAT#: STEM-CT-0207-ZJF)

The Recombinant Human Her3/ErbB3 (hFc Tag) manufactured by Yisheng is suitable for studying Her3/ErbB3-mediated cellular processes.
ErbB3, also called Her3 (human epidermal growth factor receptor 3), is a type I membrane glycoprotein and a member of the ErbB family of tyrosine kinase receptors. It is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts, and Schwann cells. Monomeric ErbB3 serves as a low-affinity receptor for the heregulins (HRG) and heterodimerizes with ErbB2 to form a high-affinity receptor complex. ErbB3 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation.

Synonyms: ErbB3; ErbB-3; HER3; HER3c-erbB-3; LCCS2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; ERBB3
Uniprot No.: P21860
Source: Recombinant Human Her3/ErbB3 Protein is expressed from HEK293 Cells with hFc tag at the C-terminal. It contains Ser20-Thr643.
Molecular Weight: The protein has a predicted MW of 95.4 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: > 95% as determined by SDS-PAGE and HPLC
Activity: ELISA Data: Immobilized Human Her3, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Her3 Antibody, hFc Tag with the EC50 of 13.5ng/ml determined by ELISA.
Endotoxin: < 1.0 EU per 1μg of the protein by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Shipped with ice pack.
Stored at -20℃ to -80℃ for 1 year.
2-7 days, 2 to 8 ℃ under sterile conditions after reconstitution.
3 months, -20 ℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
For safety and health, please wear lab coats and disposable gloves for operation.

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