Recombinant Human CD96 Protein, Seafrom (CAT#: STEM-CT-0136-ZJF)

The Recombinant Human CD96 Protein manufactured by Seafrom is suitable for immune cell activation and regulation.
The progression of pancreatic cancer (PC) is significantly associated with tumor immune escape, possibly linked to dysfunction in natural killer (NK) cell activity. CD226, CD96, and TIGIT share the ligand CD155 and play crucial roles in regulating NK cell function. This study aims to explore the roles of these molecules in NK cells of PC patients. TIGIT and CD96, along with the co-stimulatory receptor CD226, form a pathway resembling the CD28/CTLA-4 pathway, sharing ligands and distinct receptors. The ligand affinity in this pathway finely tunes immune responses. Although the roles of TIGIT and CD96 as immune checkpoint receptors in T cells and NK cells are just beginning to be understood, accumulating data support their targeted use to enhance anti-tumor immune responses. A clear understanding of the immune cell populations regulated by TIGIT and CD96 is crucial for the design of immunotherapies that combine these receptors with existing immune checkpoint blockade therapies. Dysfunction of CD96 may lead to C syndrome, a rare syndrome characterized by multisystem defects, including trigonocephaly, developmental delay, cardiac defects, and distinctive facial features. Regarding storage information for lyophilized products: they can be stored at 4°C for 24 months, and the liquid after reconstitution can be stored at -20°C for 6-12 months, avoiding repeated freeze-thaw cycles.

Expression Host: Human HEK293 cells
Source: Human
Synonyms: TACTILE
Protein Sequence: The DNA sequence encodes human CD96 (UniProtKB/Swiss-Prot: P40200.2), expressed with a His tag at the C-terminus.
Molecular Weight: Recombinant human CD96 protein contains 482 amino acids, with a predicted theoretical molecular weight of 55.2 kD.
Purity: ≥95%, confirmed by SDS-PAGE gel and high-performance liquid chromatography (HPLC) analysis.
Endotoxin: <0.1 EU/μg
Biological Activity: Immobilized human CD155 (Fc tag) at 2 μg/mL (100 μL/well) can bind to human CD96 (His tag) in a linear range of 0.2-1.5 μg/mL.
Purification Method: Chromatographic purification
Composition: Sterile PBS (pH 7.4) freeze-dried powder containing 6% mannitol.

Optimal Stability
Gmp-Grade Quality
Low Batch-to-Batch Variability
Exceptional Specificity
Robust Bioactivity
Efficient and Reliable Performance

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