Recombinant Murine EG-VEGF Protein, Seafrom (CAT#: STEM-CT-0060-ZJF)

The Recombinant Murine EG-VEGF Protein manufactured by Seafrom is suitable for angiogenesis and vascular development studies.
EG-VEGF, or Endocrine Gland-Derived Vascular Endothelial Growth Factor, is a secreted angiogenic mitogenic growth factor expressed in the steroidogenic glands, ovary, testis, adrenal gland, and placenta. EG-VEGF induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. The murine EG-VEGF gene codes for a 105 amino acid polypeptide containing an N-terminal signal sequence of 19 amino acids.
The lyophilized formulation can be stored at 2-8°C for 24 months. If further dilution is required, it can be reconstituted with injection-grade water, physiological saline, culture medium, or PBS. Once reconstituted, it should be aliquoted, and the aliquots should be stored at -20°C for 6 months or at -80°C for 12 months, with a post-dilution concentration not less than 100 μg/mL, while avoiding repeated freeze-thaw cycles.

Expression Host: HEK293 Cells
Synonyms: Endocrine Gland-derived Vascular Endothelial Growth Factor, Prokineticin 1, PROK1
Protein Sequence: DNA sequence encodes mouse EG-VEGF (NCBI: NP_001037847.1) with an Fc tag at the C-terminus, contains 319 amino acids, predicted molecular weight of 36.6 kD
Purity: >90%, analyzed by SDS-PAGE
Endotoxin Level: <1 EU/μg
Biological Activity: Assessed by EJG bovine adrenal endothelial cell proliferation with an ED50 of 1-4 μg/mL
Purification Method: Chromatographic purification
Appearance: White loose body

Stable Cell Library
Large-Scale Expression
High Activity
Batch Stability
Gmp-Quality Control
Ensured Product Quality

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