Masson Stainning for connective tissue (CAT#: STEM-PET-0001-WXH)

Introduction

Masson's trichrome staining is the most classic method in connective tissue staining, also known as Masson's staining, is one of the main methods to display fibers in tissues, and is an authoritative and classic technical method for collagen fiber staining. After staining, collagen fibers are blue (aniline blue counterstaining) or green (bright green counterstaining), muscle fibers and cellulose are red, and red blood cells are orange red.<br />The dyeing principle of this method is related to the size of anionic dye molecules and the penetration of tissues. Molecular size is reflected by molecular weight. Small molecular weight can easily penetrate into tissues with dense structure and low permeability; while large molecular weight can only enter tissues with loose structure and high permeability. However, the molecular weight of light green or aniline blue is very large, so after Masson staining, the muscle fibers are red, and the collagen fibers are green or blue, which is mainly used to distinguish collagen fibers and muscle fibers.