Immunodiffusion to detect antibodies and antigen (CAT#: STEM-MB-0182-WXH)

The basis for the immunodiffusion test is concurrent migration of antigen and antibody towards each other through an agar or agarose gel. As the antigen and antibody come in contact, they combine to form a precipitate that is trapped in the gel matrix and produces a visible line.
The commonly known types are: Single diffusion in one dimension, Double diffusion in one dimension, Single diffusion in two dimension (radial immunodiffusion or Mancini method), Double diffusion in two dimensions.

Detect antibodies and antigen.

1. Make Agarose.
2. Cool the solution down to 55-60oC. Let the gel be set for 30 minutes.
3. The glass plate should be placed on the template supplied.
4. Punch wells using the aid of the gel puncher that is corresponding to the marking of the
template.
5. Add antiserum as well as the antigens that are corresponding to it into the wells.
6. Place the glass plate in a humid chamber for the night at 37C.
7. After incubation,look for precipitin lines that are opaque between the antigen and antiserum wells.