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Rabbit Monoclonal Antibody Production (CAT#: STEM-MB-0117-WXH)
Introduction
Rabbit monoclonal antibody has higher affinity and recognizes more kinds of epitopes than mouse monoclonal antibody; it can recognize antigens that do not produce immunity in mice; it makes high-throughput screening of fusion cells possible.
Advantages of rabbit monoclonal antibodies include:
1. High affinity
2. High specificity and sensitivity
3. Can recognize more novel epitopes
4. More hybridoma clones
5. Can bind human and mouse proprotein at the same time
Applications
• Biomarker Development
• Immunohistochemical clinical diagnosis of cancer
• Development of Antibody Therapeutics
• Immunohistochemical clinical diagnosis of cancer
• Development of Antibody Therapeutics
Procedure
1. Animal immunity;
2. Collect blood B lymphocytes or bone marrow spleen, extract mRNA, and prepare cDNA by reverse transcription;
3. Use PCR and enzyme-cut ligation methods to connect antibody genes into phagemid vectors, transform bacteria, and construct antibody libraries;
4. Use various methods such as solid-phase screening to conduct affinity screening to screen high-affinity rabbit monoclonal antibodies;
5. Adopt high-efficiency eukaryotic expression system to secrete and express rabbit monoclonal antibody;
6. Identify the binding activity of rabbit antibody to antigen.
2. Collect blood B lymphocytes or bone marrow spleen, extract mRNA, and prepare cDNA by reverse transcription;
3. Use PCR and enzyme-cut ligation methods to connect antibody genes into phagemid vectors, transform bacteria, and construct antibody libraries;
4. Use various methods such as solid-phase screening to conduct affinity screening to screen high-affinity rabbit monoclonal antibodies;
5. Adopt high-efficiency eukaryotic expression system to secrete and express rabbit monoclonal antibody;
6. Identify the binding activity of rabbit antibody to antigen.
Notes
Customers provide antigen or antigen sequences.
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