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Yeast Expression System (CAT#: STEM-MB-0124-WXH)

Introduction

Yeast expression system is the most economical and efficient eukaryotic protein expression system, which can successfully achieve intracellular expression or secreted expression, and its amplification medium is relatively cheap, and the culture conditions are not demanding, so it is suitable for industrial amplification. Like the mammalian cell expression system, the yeast protein expression system can modify the expressed protein such as glycosylation, acylation, lipidation, phosphorylation, etc. to ensure the natural conformation of the protein. It can be used to prepare protein raw materials with high added value that are very close to natural proteins.




Applications

It is suitable for expressing eukaryotic proteins, secreted proteins, certain cytoplasmic localized proteins without glycosylation modification, etc.

Procedure

1. Target gene synthesis and codon optimization
2. Subcloning the gene of interest
3. Construction of linearized expression plasmids
4. Recombinant expression plasmid
5. Electroporation of expression strains
6. PCR identification and screening of high-copy expression strains
7. Protein induced expression and optimized expression conditions
8. Separation, purification and detection of target protein

Notes

Customers provide sequence information or plasmids of target proteins.

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