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Single-chain antibody (scFv) is a small molecule composed of the variable region of the antibody heavy chain and the variable region of the light chain connected by a peptide chain, and is the smallest functional structural unit with antibody activity . Single-chain antibodies can be expressed by expression systems, and can also be prepared by phage display technology. <br />scFv has the advantages of small molecular weight, strong penetrating power, and high specificity, and has important applications in targeted therapy, imaging diagnosis, intracellular immunity, and biological detection.