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Lumit™ Immunoassays to quickly detect antigens and antibodies (CAT#: STEM-MB-0178-WXH)

Introduction

Lumit™ Technology enables bioluminescence-based detection of specific antigens or antibodies, simplifying immunoassay protocols and speeding time to results. Based on the award-winning NanoBiT® complementation system, Lumit™ Technology powers sensitive and accurate immunoassays for research and clinical applications.<br />Lumit™ Immunoassays provide a faster, more reliable alternative to ELISAs, eliminating the variability associated with multiple wash steps, and providing the convenience of a simple and sensitive luminescent detection method. Because of these advantages, Lumit™ Immunoassays are easily adaptable to automation for labs processing large numbers of samples.




Principle

Lumit™ Immunoassays are based on NanoBiT® technology—a protein complementation system that uses two subunits of an engineered luciferase enzyme: small bit (SmBiT) and large bit (LgBiT). These two subunits have low affinity for each other and only can form an active NanoLuc® luciferase when in close proximity.

Applications

Quantify target analytes (antigens, antibodies) in cell culture samples.