Turbidimetric Immunoassay to quantify antigen-antibody complexes. (CAT#: STEM-MB-0176-WXH)

Introduction

Turbidimetric immunoassay, also known as immunoturbidimetry or turbidimetry, is based on measuring the turbidity of a sample to determine the level of an analyte. This method is commonly used to quantify antigen-antibody complexes. This assay has been widely used in clinical diagnosis, e.g., measurement of PAI-1 and serum C-reactive protein (CRP).<br />Latex-enhanced immunoturbidimetry (LETIA) is an optimization for conventional immunoturbidimetry. In LETIA, inert microscopic particles amplify the immune complexes, significantly increasing the sensitivity of the reaction and detection.

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Principle Applications Procedure Materials Notes Related Products

Principle

The formation of antigen-antibody complex increases the turbidity of the sample. When light passes through the reaction solution, part of the light is scattered by the sample, part of the light is absorbed by the sample, and the rest of the light passes through the sample. The concentration of protein antigen in the sample is determined by measuring the absorbance of light from a sample.

Applications

• Determine the concentration of protein antigen.
• Clinical diagnosis.

Procedure

1. Calibration
2. Quality control
3. Sample testing

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