Cytometric Bead Array (CBA) (CAT#: STEM-CBT-0055-WXH)

Introduction

Cytometric bead array (CBA) is a flow cytometry application that allows users to quantify multiple proteins simultaneously. Compared to other quantifier assays, as enzyme-linked immunosorbent assay (ELISA) and Western blot, CBA significantly reduces sample requirements and time to results.<br />This technology allows for the design and creation of assays to measure a variety of analytes including inflammatory mediators, chemokines, immunoglobulin isotypes, intracellular signaling molecules, apoptotic mediators, adhesion molecules, and antibodies. <br />This new technology allows for (1) evaluation of multiple analytes in a single<br />sample; (2) utilization of minimal sample volumes to glean data; (3) reproducibility and results comparative with previous experiments;<br />(4) direct comparison with existing assays; and (5) a more rapid evaluation of multiple samples in a single platform.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

The basic idea behind the bead-based capture array technology is that each type of bead has a specific protein-capturing antibody conjugated on to its surface. Each antibody has its own specific fluorescent intensity. Sometimes, you can even sort the beads by size. In a single experiment, you incubate your sample with a variety of beads to detect a wide spectrum of proteins of interest. Based on the fluorescence intensity of the beads (or size), you can determine the specific protein that each bead captured.

Applications

• Oncology research and monitoring of adjuvant diagnosis, efficacy and prognosis of oncological diseases.
• Stem cell research and monitoring of stem cell therapy.
• Immunology research and ancillary diagnosis and monitoring of immune diseases.
• Infectious disease research and ancillary diagnosis and efficacy evaluation.
• Immune function monitoring.
• Drug efficacy evaluation, drug development, vaccine research.
• Quantitative analysis of phosphorylated proteins and apoptosis-related proteins in cell signaling.

Procedure

1. Sample & Standard Preparation
2. Incubation of the sample with capture beads
3. Labeled fluorescent detection reagent
4. Washing
5. Flow cytometry assay
6. Data analysis

Materials

FITC, PE label

Other recommended products

In Vivo Flow Cytometry (IVFC) (CAT#: STEM-CBT-0051-WXH)
Imaging Flow Cytometry (IFC) (CAT#: STEM-CBT-0053-WXH)
Analysis of Membrane potential by Flow Cytometry (CAT#: STEM-CBT-0068-WXH)
Multicolour Flow Cytometry (CAT#: STEM-CBT-0050-WXH)
Analysis of Cell Proliferation by Flow Cytometry (CAT#: STEM-CBT-0058-WXH)
Measurement of Intracellular pH by Flow Cytometry (CAT#: STEM-CBT-0056-WXH)
Analysis of Autophagy by Flow Cytometry (CAT#: STEM-CBT-0059-WXH)
Cell Counting by Flow Cytometry (CAT#: STEM-CBT-0072-WXH)