High-quality products
Reliable performance
- Home
- Technical Services
- Cell Technology
- Flow Cytometry Technology
- Normal Applications of Flow Cytometry
Analysis of Cell Proliferation by Flow Cytometry (CAT#: STEM-CBT-0058-WXH)
Introduction
Assays that measure cell proliferation in individual cells work by monitoring changes in intracellular markers and/or DNA. Flow cytometry is an ideal platform for measuring cell proliferation and enables us to study various unique cell types within a heterogeneous population.
Cell proliferation can be measured by flow cytometry using several different assays and markers.
Principle
These assays use different methods to target proliferation-related events such as:
1. Incorporation of thymidine analogs (BrdU) into replicating DNA
2. Generational tracking of inheritable permanent dyes (CFSE)
3. Expression of proliferation related antigens (Ki67, PCNA)
1. Incorporation of thymidine analogs (BrdU) into replicating DNA
2. Generational tracking of inheritable permanent dyes (CFSE)
3. Expression of proliferation related antigens (Ki67, PCNA)
Applications
• Determine the influence of the drug on cell growth and proliferation.
• Support investigations into immunophenotyping, compound toxicity, drug effects on tumor cell growth, and T cell behavior under different conditions.
• Determine the influence of the drug on apoptpsis.
• Support investigations into immunophenotyping, compound toxicity, drug effects on tumor cell growth, and T cell behavior under different conditions.
• Determine the influence of the drug on apoptpsis.
Procedure
1. Culture medium preparation
2. Mononuclear cell isolation
3. Cell staining
4. Incubate for desired length of time under growth conditions
5. Analyze using a flow cytometer
2. Mononuclear cell isolation
3. Cell staining
4. Incubate for desired length of time under growth conditions
5. Analyze using a flow cytometer
Materials
Ethynyl Deoxyuridine
BrdU (thymidine analogs)
CFSE (inheritable permanent dyes)
Expression of proliferation related antigens (Ki67, PCNA)
BrdU (thymidine analogs)
CFSE (inheritable permanent dyes)
Expression of proliferation related antigens (Ki67, PCNA)
Other recommended products
-
Analysis of Proteins Interactions by Flow Cytometry with FRET (CAT#: STEM-CBT-0065-WXH)
-
Analysis of DNA Content by Flow Cytometry (CAT#: STEM-CBT-0062-WXH)
-
Imaging Flow Cytometry (IFC) (CAT#: STEM-CBT-0053-WXH)
-
Analysis of Reactive Oxygen Species(ROS) by Flow Cytometry (CAT#: STEM-CBT-0071-WXH)
-
Mass Cytometry (CAT#: STEM-CBT-0052-WXH)
-
Analysis of Cytokines by Flow Cytometry (CAT#: STEM-CBT-0066-WXH)
-
Analysis of Signal Transduction by Phospho Flow Cytometry (CAT#: STEM-CBT-0069-WXH)
-
Analysis of Autophagy by Flow Cytometry (CAT#: STEM-CBT-0059-WXH)
Wide range of applications
For various laboratory needs
Expert support
Professional service team
Fast delivery
Global shipping