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Fluorescence Activated Cell Sorting (FACS) by Flow Cytometry (CAT#: STEM-CBT-0070-WXH)

Introduction

Fluorescence-activated cell sorting (FACS) is a specialized type of flow cytometry. It provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell. It is a useful scientific instrument, as it provides fast, objective and quantitative recording of fluorescent signals from individual cells as well as physical separation of cells of particular interest. This method enables researchers to better understand the characteristics of a single cell population without the influence of other cells.




Principle

FACS technology separates cells based on cell surface markers. Antigenic ligands, such as proteins and carbohydrates, give each cell a unique surface phenotype, and specific antibodies associated with the cell surface antigens are then used to target cells with those antigens.

Applications

• Cancer Research--isolate cancer cells from a mixed population of cells.
• Immunology Research--study the immune system.
• Stem Cell Research.
• Developmental Biology Research.
• Stem cell tissue engineering research.
• Cell proliferation, activity and apoptosis research.
• Study of disease cell genesis, development and transmigration such as inflammatory cells, tumor cells, etc..
• Gene expression and expression regulation.
• Intracellular signaling and biomolecular interactions.
• Research on pathogenesis and pathogenicity of various pathogens such as viruses and bacteria.
• Pharmacological and pharmacodynamic research and drug development.
• Transplantation and cell therapy research.
• Effect of biomaterials on cell structure, activity and function.

Procedure

1.Label the cells with fluorescent dyes
2.Cells are washed and diluted
3.Detected by flow cytometer

Materials

PE,FITC