Measurement of Intracellular pH by Flow Cytometry (CAT#: STEM-CBT-0056-WXH)

Introduction

Intracellular pH (pHi) plays many critical roles in cell, enzyme, and tissue activities, including proliferation and apoptosis, multidrug resistance (MDR), ion transport, endocytosis, and muscle contraction.Monitoring pH changes inside living cells is also important for studying cellular internalization pathways, such as phagocytosis, endocytosis, and receptor ligand internalization. Changes of pHi effect the nervous system too, by influencing synaptic transmission, neuronal excitability, cell-cell coupling via gap junctions, and signal cascades. Abnormal pHi values are associated with inappropriate cell function, growth, and division and are observed in some common disease types such as cancer and Alzheimer’s.