Analysis of Creatine kinase (CK) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1347-LGZ)

Phosphocreatine kinase (CK, EC 2.7.3.2) catalyzes creatine phosphate and ADP to form creatine and ATP, and hexokinase (HK) catalyzes creatine and glucose to form glucose 6-phosphate. Glucose-6-phosphate dehydrogenase catalyzes Glucose 6-phosphate with NADP+ to generate NADPH, resulting in increased light absorption at 340 nm wavelength.

It is used to detect creatine kinase activity in serum, plasma, animal tissues and cells.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum, plasma, animal tissues and cells