Analysis of Biopharmaceutical Lyophilizate Residual Moisture by Loss on drying (LOD) Method (CAT#: STEM-B-0415-CJ)

Dry weightlessness (LOD) is the simplest and oldest residual moisture analysis technique and can be performed without any specialized laboratory equipment. Remove the sample from the vial and weigh it into a dry container. Dry the sample using heat, vacuum or desiccant to remove moisture, then cool and reweigh. The moisture content is calculated by dividing the weight lost during drying by the original weight. While this method is simple, it lacks the accuracy and specificity required for most applications. Dry weight loss test only obtained the amount of weight loss, not specific to water.

Biopharmaceutica

1. Remove the sample from the vial and weigh it into a dry container. 2. Dry the sample using heat, vacuum or desiccant to remove moisture, then cool and reweigh.
3. Calculate the moisture content by dividing the weight lost during drying by the original weight .

• Sample: Proteins, Peptides, Nucleic acids, Naturally-occurring compounds & more

• Other volatiles in the product (e.g., ethanol) may also be repelled, producing inaccurate results.
• Sample degradation may occur at higher temperatures, which may lead to erroneous high results, especially for proteins.
• ncomplete sample drying may produce erroneous low results.