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T-banding (CAT#: STEM-GT-0014-WXH)
Introduction
The T-banding is a modified reverse chromosomal banding technique wherein the telomeres are strongly stained as the faint R-banding still occurs over the rest of the chromosome.
Applications
To study the telomeric region of chromosomes.
Stains only the end of chromosomes.
Helps preliminarily the end replication process.
Helps in studying other abnormalities associated with telomeres.
Stains only the end of chromosomes.
Helps preliminarily the end replication process.
Helps in studying other abnormalities associated with telomeres.
Procedure
1. Prepare slides from a good harvested cell suspension and age it for a week.
2. Air-dry it and place slides in PBS solution for 20 to 60 minutes at 87°C temperature.
3. Wash slides in PBS.
4. Stain slides in 3% Giemsa solution or acridine orange dye for 5 to 20 minutes.
5. Stain slides in Hoechst 33258 for few minutes.
6. Air dry to slides and observe under the microscope.
2. Air-dry it and place slides in PBS solution for 20 to 60 minutes at 87°C temperature.
3. Wash slides in PBS.
4. Stain slides in 3% Giemsa solution or acridine orange dye for 5 to 20 minutes.
5. Stain slides in Hoechst 33258 for few minutes.
6. Air dry to slides and observe under the microscope.
Materials
Microscope, coverslip and slides, PBS solution, Giemsa stain, Hoechst 33258, UV lamp and acridine orange dye.
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