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array Comparative Genomic Hybridisation (aCGH) (CAT#: STEM-GT-0017-WXH)
Introduction
In an attempt to overcome some of the limitations associated with traditional CGH, investigators have developed a newer method that combines the principles of CGH with the use of microarrays. Instead of using metaphase chromosomes, this method—which is known as array CGH, or simply aCGH—uses slides arrayed with small segments of DNA as the targets for analysis.
Principle
These microarrays are created by the deposit and immobilization of small amounts of DNA (known as probes) on a solid support, such as a glass slide, in an ordered fashion. Probes vary in size from oligonucleotides manufactured to represent areas of interest (25–85 base pairs) to genomic clones such as bacterial artificial chromosomes (80,000–200,000 base pairs). Because probes are several orders of magnitude smaller than metaphase chromosomes, the theoretical resolution of aCGH is proportionally higher than that of traditional CGH.
Applications
Simultaneously detect aneuploidies, deletions, duplications, and/or amplifications of any locus represented on an array.
Studying Specific Chromosomal Regions.
Studying Specific Chromosomal Regions.
Procedure
1. Test DNA and control DNA are labeled with fluorescent dyes and applied to the microarray.
2. Test DNA and control DNA compete to attach, or hybridize to the microarray.
3. The microarray scanner measures the fluorescent signals.
4. Analyze the data and generates a plot.
2. Test DNA and control DNA compete to attach, or hybridize to the microarray.
3. The microarray scanner measures the fluorescent signals.
4. Analyze the data and generates a plot.
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