Endotoxin Removal (CAT#: STEM-MB-0128-WXH)

Introduction

Endotoxin is a component in the cell wall of Gram-negative bacteria, which is toxic to the host. Therefore, it is necessary to detect and control the content of endotoxin to avoid adverse reactions of the host when the recombinant protein is used in animal experiments, which will affect the experimental results.<br />Due to the small size of bacterial endotoxins, strong heat resistance and chemical stability, general filtration, heating and chemical methods are not easy to remove or inactivate endotoxins. The more commonly used endotoxin removal methods are mostly ultrafiltration or affinity chromatography. The lower limit of ultrafiltration membrane pore size (5-100nm) is similar to bacterial endotoxin, so the ultrafiltration membrane with small pore size can be used to remove bacterial endotoxin.

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Principle Applications Procedure Materials Notes Related Products

Applications

Prevent endotoxin from affecting cell experiments and animal experiments

Procedure

1. Ultrafiltration method: use the difference in molecular weight between endotoxin and other substances to remove endotoxin. one
2. Activated carbon method: This method is mainly suitable for the removal of endotoxins in small molecule solutions with simple components such as water. Because of its poor selectivity and easy adsorption of active ingredients, it is generally less used. This method can be used in combination with super method to remove endotoxin better
3. Heat treatment method: This method is mainly used for the removal of endotoxin in heat-resistant items.
4. Chemical degradation method: use strong acid, strong alkali or oxidant to degrade endotoxin itself, thereby removing endotoxin.
5. Affinity membrane separation system method: This method is based on affinity chromatography, using cellulose membrane as the basic material, and bonding different ligands through chemical methods. These ligands have a strong effect on endotoxin molecules or aggregates. of adsorption.

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